Structure of the human neutral amino acid transporter ASCT2 in complex with nanobody 469
Resolution: 2.31 Å
EM Method: Single-particle
Fitted PDBs: 8oud
Q-score: 0.666
Khare S, Villalba MI, Canul-Tec JC, Cajiao AB, Kumar A, Backovic M, Rey FA, Pardon E, Steyaert J, Perez C, Reyes N
Nat Struct Mol Biol (2024) [ Pubmed: 38671230 DOI: doi:10.1038/s41594-024-01295-6 ]
- Nanobody 69 (Complex from Lama glama)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody 469 (13 kDa, Protein from Lama glama)
- Alanine serine cysteine transporter 2 (Complex from Homo sapiens)
- Neutral amino acid transporter b(0) (56 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Alanine (89 Da, Ligand)
- Complex of human asct2 with nanobody 69 (200 kDa, Complex)
Complex of human ASCT2 with Syncytin-1
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 8ouh
Q-score: 0.553
Khare S, Villalba MI, Canul-Tec JC, Cajiao AB, Kumar A, Backovic M, Rey FA, Pardon E, Steyaert J, Perez C, Reyes N
Nat Struct Mol Biol (2024) [ Pubmed: 38671230 DOI: doi:10.1038/s41594-024-01295-6 ]
- Syncytin-1 (49 kDa, Protein from Homo sapiens)
- Syncytin-1 (Complex from Homo sapiens)
- Neutral amino acid transporter b(0) (58 kDa, Protein from Homo sapiens)
- Alanine serine cysteine transporter 2 (Complex from Homo sapiens)
- Complex of asct2 with synctin-1 (180 MDa, Complex)
- Alanine (89 Da, Ligand)
Complex of ASCT2 with Suppressyn
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 8oui
Q-score: 0.435
Khare S, Villalba MI, Canul-Tec JC, Cajiao AB, Kumar A, Backovic M, Rey FA, Pardon E, Steyaert J, Perez C, Reyes N
Nat Struct Mol Biol (2024) [ Pubmed: 38671230 DOI: doi:10.1038/s41594-024-01295-6 ]
- Neutral amino acid transporter b(0) (57 kDa, Protein from Homo sapiens)
- Alanine (89 Da, Ligand)
- Complex of alanine serine cysteine transporter 2 with suppressyn (Complex from Homo sapiens)
- Suppressyn (18 kDa, Protein from Homo sapiens)
Heterotrimeric Complex of Human ASCT2 with Syncytin-1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8ouj
Q-score: 0.339
Khare S, Villalba MI, Canul-Tec JC, Cajiao AB, Kumar A, Backovic M, Rey FA, Pardon E, Steyaert J, Perez C, Reyes N
Nat Struct Mol Biol (2024) [ Pubmed: 38671230 DOI: doi:10.1038/s41594-024-01295-6 ]
- Syncytin-1 (49 kDa, Protein from Homo sapiens)
- Syncytin-1 (Complex from Homo sapiens)
- Neutral amino acid transporter b(0) (58 kDa, Protein from Homo sapiens)
- Complex asct2 with syncytin-1 (180 MDa, Complex)
- Alanine serine cysteine transporter 2 (Complex from Homo sapiens)
- Alanine (89 Da, Ligand)
ACE2-B0AT1 complex bound with glutamine
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8i92
Q-score: 0.452
Li Y, Chen Y, Zhang Y, Shen Y, Xu K, Liu Y, Wang Z, Yan R
Cell Discov (2023) 9 pp. 93-93 [ Pubmed: 37684251 DOI: doi:10.1038/s41421-023-00596-2 ]
- Zinc ion (65 Da, Ligand)
- Angiotensin-converting enzyme 2 (95 kDa, Protein from Homo sapiens)
- Ace2-b0at1 complex bound with glutamine (Complex from Homo sapiens)
- Sodium-dependent neutral amino acid transporter b(0)at1 (68 kDa, Protein from Homo sapiens)
- Glutamine (146 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
ACE2-B0AT1 complex bound with methionine
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8i93
Q-score: 0.474
Li Y, Chen Y, Zhang Y, Shen Y, Xu K, Liu Y, Wang Z, Yan R
Cell Discov (2023) 9 pp. 93-93 [ Pubmed: 37684251 DOI: doi:10.1038/s41421-023-00596-2 ]
- Zinc ion (65 Da, Ligand)
- Ace2-b0at1 complex bound with methionine (Complex from Homo sapiens)
- Sodium-dependent neutral amino acid transporter b(0)at1 (73 kDa, Protein from Homo sapiens)
- Methionine (149 Da, Ligand)
- Angiotensin-converting enzyme 2 (86 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
ACE2 -Targeting Monoclonal Antibody as Potent and Broad-Spectrum Coronavirus Blocker
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7v61
Q-score: 0.366
Chen Y, Zhang YN, Yan R, Wang G, Zhang Y, Zhang ZR, Li Y, Ou J, Chu W, Liang Z, Wang Y, Chen YL, Chen G, Wang Q, Zhou Q, Zhang B, Wang C
Signal Transduct Target Ther (2021) 6 pp. 315-315 [ Pubmed: 34433803 DOI: doi:10.1038/s41392-021-00740-y ]
- 3e8 (23 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- 3e8 (49 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- Angiotensin-converting enzyme 2 (93 kDa, Protein from Homo sapiens)
- Ace2-b0at1 complex (Complex from Homo sapiens)
- Sodium-dependent neutral amino acid transporter b(0)at1 (73 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
ASCT2 in the presence of the inhibitor Lc-BPE in the outward-open conformation.
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 7bcq
Q-score: 0.427
Garibsingh RA, Ndaru E, Garaeva AA, Shi Y, Zielewicz L, Zakrepine P, Bonomi M, Slotboom DJ, Paulino C, Grewer C, Schlessinger A
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2104093118 Pubmed: 34507995 ]
ASCT2 in the presence of the inhibitor ERA-21 in the outward-open conformation.
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 7bct
Q-score: 0.382
Garibsingh RA, Ndaru E, Garaeva AA, Shi Y, Zielewicz L, Zakrepine P, Bonomi M, Slotboom DJ, Paulino C, Grewer C, Schlessinger A
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2104093118 Pubmed: 34507995 ]
Conformation 1 of S-ACE2-B0AT1 ternary complex
Resolution: 8.3 Å
EM Method: Single-particle
Fitted PDBs: 7dwx
Q-score: 0.059
Yan R, Zhang Y, Li Y, Ye F, Guo Y, Xia L, Zhong X, Chi X, Zhou Q
Cell Res (2021) 31 pp. 717-719 [ Pubmed: 33737693 DOI: doi:10.1038/s41422-021-00490-0 ]
- Conformation 1 of s-ace2-b0at1 ternary complex (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Leucine (131 Da, Ligand)
- Water (18 Da, Ligand)
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Angiotensin-converting enzyme 2 (93 kDa, Protein from Homo sapiens)
- Sodium-dependent neutral amino acid transporter b(0)at1 (73 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)